{"id":5046,"date":"2011-02-28T00:00:00","date_gmt":"2011-02-27T23:00:00","guid":{"rendered":"https:\/\/sesc.cat\/granulomatous-disseminated-lesions-in-pig-carcasses\/"},"modified":"2022-04-02T16:22:48","modified_gmt":"2022-04-02T15:22:48","slug":"granulomatous-disseminated-lesions-in-pig-carcasses","status":"publish","type":"post","link":"https:\/\/sesc.cat\/en\/granulomatous-disseminated-lesions-in-pig-carcasses\/","title":{"rendered":"Granulomatous disseminated lesions in pig carcasses"},"content":{"rendered":"<p>Between late 2010 and early 2011, several inquiries were submittetd to SESC regarding the presence of granulomatous lesions in pig carcasses, coming from up to 5 different farms.<\/p>\n<p>The lesions were nodular, of whitish appearance and sometimes were calcified and affected the liver, spleen and lungs showing a miliary distribution. The lymph nodes were also involved (see Figures 1 to 4).<\/p>\n<p>Histopathological examination showed necrotic lesions associated with granulomatous inflammatory infiltrate with giant multinucelated cells. The lesion was diagnosed as multifocal, generalized granulomatous-necrotizing splenitis, hepatitis and lymphadenitis.<\/p>\n<p>Ziehl-Neelsen revealed, in some cases, the presence of acid-resistant bacteria.<\/p>\n<p>Lesions and the Ziehl-Neelsen staining indicated that it was a <strong>mycobacteriosis<\/strong>.<\/p>\n<p>A molecular study and microbiological culture were performed to confirm the diagnosis and determine the mycobacteria causing the lesions.<\/p>\n<p>The tests carried out were:<\/p>\n<ul>\n<li>Direct PCR of the lesioned tissue: a positive result was obtained, although weak, indicating that it might be a mycobacteria included in the <em>M. tuberculosis<\/em> complex , this complex includes species causing human and bovine tuberculosis. It has been described that the DNA IS6110 target used for identification of mycobacteria from this complex may cross-react with other mycobacteria such as <em>M.avium<\/em> complex, <em>M. intracellulare<\/em> complex and <em>M. xenopi<\/em> among others (Carridge et al. 1993).<\/li>\n<li>Microbiological culture and PCR of growing colonies: using two different decontamination methods mycobacterial growth could be isolated . PCR from these colonies confirmed that it was non-tuberculous mycobacteria (see Figure 5).<\/li>\n<li>Molecular characterization of bacteria of the genus <em>Mycobacterium<\/em>: the reference laboratory confirmed the presence of bacteria of the genus <em>Mycobacterium<\/em> and identified them as <strong><em>M.avium. subsp. avium<\/em><\/strong>.<\/li>\n<\/ul>\n<p>One possible source of the infection outbreak would be a <em>M. avium subsp. avium<\/em> contamination present in the feed of animals (fairly common pathogen in gallinaceous birds which can be excreted in large quantities through the chloaca). The fact that in most cases a clear involvement of the abdominal cavity was observed supported a digestive entry of the infectious agent.<\/p>\n<p>This case has been published in <a href=\"http:\/\/veterinaryrecord.bmj.com\/content\/174\/4\/96.1.extract\">Veterinary Record<\/a>.<\/p>\n<div id=\"attachment_293\" style=\"width: 630px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/fetge.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-293\" class=\"size-large wp-image-5931\" title=\"Miliary granulomatous liver lesions.\" src=\"https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/fetge-617x600.jpg\" alt=\"Miliary granulomatous liver lesions.\" width=\"620\" height=\"602\" srcset=\"https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/fetge-617x600.jpg 617w, https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/fetge-300x292.jpg 300w, https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/fetge-768x746.jpg 768w, https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/fetge-1536x1493.jpg 1536w, https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/fetge-2048x1990.jpg 2048w\" sizes=\"auto, (max-width: 620px) 100vw, 620px\" \/><\/a><p id=\"caption-attachment-293\" class=\"wp-caption-text\">Miliary granulomatous liver lesions.<\/p><\/div>\n<div id=\"attachment_296\" style=\"width: 630px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/LN-mesenteric.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-296\" class=\"size-large wp-image-5935\" title=\"Caseous lymphadenitis in the mesenteric lymph node.\" src=\"https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/LN-mesenteric-1024x771.jpg\" alt=\"Caseous lymphadenitis in the mesenteric lymph node.\" width=\"620\" height=\"466\" srcset=\"https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/LN-mesenteric-1024x771.jpg 1024w, https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/LN-mesenteric-300x225.jpg 300w\" sizes=\"auto, (max-width: 620px) 100vw, 620px\" \/><\/a><p id=\"caption-attachment-296\" class=\"wp-caption-text\">Caseous lymphadenitis in the mesenteric lymph node.<\/p><\/div>\n<div id=\"attachment_297\" style=\"width: 630px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/pulmo.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-297\" class=\"size-large wp-image-5937\" title=\"Granulomatous lesions in the lung parenchyma.\" src=\"https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/pulmo-1024x768.jpg\" alt=\"Granulomatous lesions in the lung parenchyma.\" width=\"620\" height=\"465\" srcset=\"https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/pulmo-1024x768.jpg 1024w, https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/pulmo-300x225.jpg 300w\" sizes=\"auto, (max-width: 620px) 100vw, 620px\" \/><\/a><p id=\"caption-attachment-297\" class=\"wp-caption-text\">Granulomatous lesions in the lung parenchyma.<\/p><\/div>\n<div id=\"attachment_298\" style=\"width: 630px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/melsa.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-298\" class=\"size-large wp-image-5939\" title=\"Miliary splenic granulomas. \" src=\"https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/melsa-1024x717.jpg\" alt=\"Miliary splenic granulomas.\" width=\"620\" height=\"434\" srcset=\"https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/melsa-1024x717.jpg 1024w, https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/melsa-300x210.jpg 300w\" sizes=\"auto, (max-width: 620px) 100vw, 620px\" \/><\/a><p id=\"caption-attachment-298\" class=\"wp-caption-text\">Miliary splenic granulomas.<\/p><\/div>\n<div id=\"attachment_299\" style=\"width: 310px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/gel-l-006-11_llegenda.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-299\" class=\"size-medium wp-image-5941\" title=\"PCR of isolated mycobacteria colonies. The banding pattern observed indicates that the growth obtained from swine samples corresponds to non tuberculous mycobacteria (only upper band). Mycobacterium bovis: culture samples from bovine tuberculosis affected cattle (double band). C +: positive control.\" src=\"https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/gel-l-006-11_llegenda-300x198.jpg\" alt=\"PCR of isolated mycobacteria colonies. The banding pattern observed indicates that the growth obtained from swine samples corresponds to non tuberculous mycobacteria (only upper band). Mycobacterium bovis: culture samples from bovine tuberculosis affected cattle (double band). C +: positive control.\" width=\"300\" height=\"198\" srcset=\"https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/gel-l-006-11_llegenda-300x198.jpg 300w, https:\/\/sesc.cat\/wp-content\/uploads\/2012\/04\/gel-l-006-11_llegenda.jpg 445w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><p id=\"caption-attachment-299\" class=\"wp-caption-text\">PCR of isolated mycobacteria colonies. The banding pattern observed indicates that the growth obtained from swine samples corresponds to non tuberculous mycobacteria (only upper band). Mycobacterium bovis: culture samples from bovine tuberculosis affected cattle (double band). C +: positive control.<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Between late 2010 and early 2011, several inquiries were submittetd to SESC regarding the presence of granulomatous lesions in pig carcasses, coming from up to 5 different farms. The lesions were nodular, of whitish appearance and sometimes were calcified and affected the liver, spleen and lungs showing a miliary distribution. The lymph nodes were also [&hellip;]<\/p>\n","protected":false},"author":6,"featured_media":5933,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[235,187,233,179],"tags":[391,397,366,399,400,410,392],"class_list":["post-5046","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-abdominal-cavity-porcine-2","category-liver-2","category-lymphoid-porcine-2","category-porcine-2","tag-abdominal-cavity-en","tag-infectious-en","tag-liver","tag-lymph-nodes-en","tag-spleen-en","tag-tb-en","tag-thoracic-cavity-en"],"acf":[],"_links":{"self":[{"href":"https:\/\/sesc.cat\/en\/wp-json\/wp\/v2\/posts\/5046","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sesc.cat\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sesc.cat\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sesc.cat\/en\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/sesc.cat\/en\/wp-json\/wp\/v2\/comments?post=5046"}],"version-history":[{"count":0,"href":"https:\/\/sesc.cat\/en\/wp-json\/wp\/v2\/posts\/5046\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sesc.cat\/en\/wp-json\/wp\/v2\/media\/5933"}],"wp:attachment":[{"href":"https:\/\/sesc.cat\/en\/wp-json\/wp\/v2\/media?parent=5046"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sesc.cat\/en\/wp-json\/wp\/v2\/categories?post=5046"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sesc.cat\/en\/wp-json\/wp\/v2\/tags?post=5046"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}